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† BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD® High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. † BD FACSFlow™ Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system. † A printed copy of the BD High Throughput Sampler User’s Guide is distributed with the BD™ High Throughput Sampler (HTS) option. This document describes how to set up and operate the HTS. It also contains a description of BD FACSDiva software features specific to the HTS. Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates. In high- throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes. † BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD® High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. † BD FACSFlow™ Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system.

LSR Fortessa . User Guide . Ragon Institute of Massachusetts General (printed version on back cover of User Guide Binder) 8. Optimizing Setup of the LSR2 system Exporting, Experiment Templates, Transferring files .

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A while back, we were recommended away from routine PRIME functions for this very reason (BD service recommendation). Now, we PRIME only when we see a need – failures or problems with QC, data shifts within an experimental run, or samples fail to flow.

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M Canto Laser Chart JF Fortessa Laser Chart. Protocols. Cell Surface Staining Protocol. BD HTS User Guide Performance. With superior optics and fluidics for maximized sensitivity and resolution, the BD LSRFortessa™ helps you analyze your sample with ease. It processes 96 samples under 15 minutes with less than 0.5% sample carryover.

A while back, we were recommended away from routine PRIME functions for this very reason (BD service recommendation).
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Flow Cytometry Service. BD LSR Fortessa SORP – How to Use Guide.

A sample coupler connects the injection port tubing on the HTS unit to the cytometer sample injection tube (SIT) (Figure 1-8 on page 22).
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High Throughput In Vitro Assessment of Latency Reversing

Setup instructions (pictur We provide different flow cytometers for your FACS analysis. Becton Dickinson LSRFortessas with up to 17 colours and HTS plate loader. Beckman Coulter prise en charge de l'option BD HTS sur les appareils BD FACSCanto, nouvelle apparence, possibilité de manuel de référence et du guide de démarrage. BD Biosciences welcomes customer input on corrections and suggestions for improvement.

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Scheduling BD LSR Fortessa SORP – How to Use Guide 3 Section 2: Switch from acquisition in plate to acquisition in tubes The standard configuration of the cytometer is to acquire samples in 96-well plates using the High Throughput Sampler (HTS). If you need to switch the configuration to acquire your samples in FACS tubes, please follow these steps: If you have the BD HTS option, connect the HTS sample coupler to the SIT and close the HTS door. Refill fluids and empty waste, if needed, and then select Cytometer > Long Clean .

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Turn on the computer. Wait for the computer to boot up. 2. Log in to the computer (facsuser, password =BDIS) and open BD FACSDiva software. Log in to Diva at the prompt with your Diva username and password. 3.

BD FACSDiva software © Becton, Dickinson and Company. This LSR Fortessa cell analyzer (BD Biosciences) uses up to five lasers (355 nm, 405 BD™ High Throughput Sampler (HTS) allows sample loading from 96-well 29 May 2014 BD FACS LSR Fortessa User Manual. Flow Cytometry Unit – Biomedical Core facility. Technion Faculty of Medicine. For help please call:.